Enzymes and Inhibitors
Thermo Scientific™ GsuI (BpmI) (5 U/μL)
The GsuI (BpmI) restriction enzyme recognizes CTGGAG(16/14)^ sites and cuts best at 30°C in B buffer (Isoschizomers: BpmI).
Thermo Scientific™ TauI (3 U/μL)
The TauI restriction enzyme recognizes GCSG^C sites and cuts best at 55°C in B buffer.
Thermo Scientific™ FastDigest BseXI
Cut at GCAGC(8/12)^ sites with Thermo Scientific™ FastDigest™ BseXI, which performs best at 65°C in 5-15 minutes using universal FastDigest Buffer (Isoschizomers: BbvI, BstV1I).
Thermo Scientific™ KpnI Buffer (10X)
Optimize digestions with KpnI restriction enzyme using this buffer, premixed with BSA for enhanced stability.
MilliporeSigma™ PvuRts1I (5-hmC Restriction Endonuclease)
PvuRts1I is an enzyme that can specifically digest DNA containing 5-hydroxymethylcytosine (5-hmC). This restriction endonucleaseis used to analyze 5-hmC patterns genomic DNA by cleaving both glucosylated and non-glucosylated 5-hmC DNA sequences.
Thermo Scientific™ SatI (Fnu4HI) (10 U/μL)
The SatI (Fnu4HI) restriction enzyme recognizes GC^NGC sites and cuts best at 37°C in G buffer (Isoschizomers: Fnu4HI, Fsp4HI, ItaI).
Thermo Scientific™ Cfr13I (Sau96I) (10 U/μL)
Cut at G^GNCC sites with Cfr13I (Sau96I) restriction enzyme, which performs best at 37°C in Tango buffer (Isoschizomers: AspS9I, BmgT120I, PspPI, Sau96I).
Thermo Scientific™ DraI, HC (50 U/μL)
Thermo Scientific™ DraI is available as a FastDigest™ enzyme which is used for rapid DNA digestion.
Thermo Scientific™ XbaI, HC (50 U/μL)
Cut at T^CTAGA sites with XbaI restriction enzyme, which functions best at 37°C in Tango buffer.
Thermo Scientific™ BfmI (SfcI) (10 U/μL)
Cut at C^TRYAG sites with BfmI (SfcI) restriction enzyme, which performs best at 37°C in Tango buffer (Isoschizomers: BstSFI, SfcI).
Thermo Scientific™ Eco57I (AcuI) (5 U/μL)
The Eco57I (AcuI) restriction enzyme recognizes CTGAAG(16/14)^ sites and cuts best at 37°C in G (+SAM) buffer (Isoschizomers: AcuI).
Invitrogen™ Anza™ 15 XmaJI
Simplify traditional restriction enzyme cloning with the Invitrogen™ Anza™ Restriction Enzyme Cloning System.
Invitrogen™ Anza™ 87 Eco47I
Simplify traditional restriction enzyme cloning with the Invitrogen™ Anza™ Restriction Enzyme Cloning System.
Thermo Scientific™ FastDigest Eco88I
Cut at C^YCGRG sites with Thermo Scientific™ FastDigest™ AvaI (Eco88I), which performs best at 37°C in 5-15 minutes using universal FastDigest Buffer.
Thermo Scientific™ BamHI-Lsp1109I Buffer (10X)
Optimize digestions with BamHI and Lsp1109I restriction enzymes with this buffer, premixed with BSA for enhanced stability.